Human anti-SARS-CoV2(N) IgG ELISA Kit
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- Min.Order:100 Vial(s)
- Production Capacity:in stock
- Payment Terms:T/T
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Business Type:Manufacturer
Country/Region:China
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FineTest Biotech Inc.
We are professional supplier of ELISA Kits,Recombinant Proteins,Antibody(Primary and secondary antibody),Reagents,Flow Cytometry Antibody.
Business Type:Manufacturer
Country/Region:China
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This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-SARS-CoV2 (N) IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-SARS-CoV2 (N) IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.